HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Papers In Press, published online ahead of print December 1, 2007
J. Lipid Res., doi:10.1194/jlr.M700359-JLR200

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M700359-JLR200v1 48/12/2751    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Listenberger, L. L. Articles by Brown, D. A. Search for Related Content PubMed PubMed Citation Articles by Listenberger, L. L. Articles by Brown, D. A. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 48, 2751-2761, December 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Adipocyte differentiation-related protein reduces the lipid droplet association of adipose triglyceride lipase and slows triacylglycerol turnover

Laura L. Listenberger^*, Anne G. Ostermeyer-Fay^*, Elysa B. Goldberg, William J. Brown and Deborah A. Brown^1,*

^* Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 18483

The online version of this article (available at http://www.jlr.org) contains supplementary data in the form of three figures.

Published, JLR Papers in Press, September 13, 2007.

¹ To whom correspondence should be addressed. e-mail: deborah.brown{at}sunysb.edu

Although neutral lipid storage droplets are ubiquitous in eukaryotic cells, very little is known about how their synthesis and turnover are controlled. Adipocyte differentiation-related protein (ADRP; also known as adipophilin) is found on the surface of lipid droplets in most mammalian cell types. To learn how ADRP affects lipid storage, we stably expressed the protein in human embryonic kidney 293 (HEK 293) cells, which express little endogenous ADRP. As expected, ADRP was targeted to the surface of lipid droplets and caused an increase in triacylglycerol (TAG) mass under both basal and oleate-supplemented conditions. At least part of the increased mass resulted from a 50% decrease in the rate of TAG hydrolysis in ADRP-expressing cells. Furthermore, ADRP expression increased the fraction of total cellular TAG that was stored in lipid droplets. ADRP expression induced a striking decrease in the association of adipose triglyceride lipase (ATGL) and mannose-6-phosphate receptor tail-interacting protein of 47 kDa with lipid droplets and also decreased the lipid droplet association of several other unknown proteins. Transient expression of ADRP in two other cell lines also reduced the lipid droplet association of catalytically inactive ATGL. We conclude that the reduced lipid droplet association of ATGL and/or other lipases may explain the decrease in TAG turnover observed in ADRP-expressing HEK 293 cells.

Supplementary key words adipophilin • PAT proteins • ADRP • ADFP • PLIN2

Abbreviations: ADRP, adipocyte differentiation-related protein; ATGL, adipose triglyceride lipase; BHK, baby hamster kidney; GFP, green fluorescent protein; HEK 293, human embryonic kidney 293; HSL, hormone-sensitive lipase; TAG, triacylglycerol; TIP47, mannose-6-phosphate receptor tail-interacting protein of 47 kDa; 293/ADRP, ADRP-expressing HEK 293

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Kaczocha, S. T. Glaser, J. Chae, D. A. Brown, and D. G. Deutsch Lipid Droplets Are Novel Sites of N-Acylethanolamine Inactivation by Fatty Acid Amide Hydrolase-2 J. Biol. Chem., January 22, 2010; 285(4): 2796 - 2806. [Abstract] [Full Text] [PDF]

				H. Wang, L. Hu, K. Dalen, H. Dorward, A. Marcinkiewicz, D. Russell, D. Gong, C. Londos, T. Yamaguchi, C. Holm, et al. Activation of Hormone-sensitive Lipase Requires Two Steps, Protein Phosphorylation and Binding to the PAT-1 Domain of Lipid Droplet Coat Proteins J. Biol. Chem., November 13, 2009; 284(46): 32116 - 32125. [Abstract] [Full Text] [PDF]

				R. Minnaard, P. Schrauwen, G. Schaart, J. A. Jorgensen, E. Lenaers, M. Mensink, and M. K.C. Hesselink Adipocyte Differentiation-Related Protein and OXPAT in Rat and Human Skeletal Muscle: Involvement in Lipid Accumulation and Type 2 Diabetes Mellitus J. Clin. Endocrinol. Metab., October 1, 2009; 94(10): 4077 - 4085. [Abstract] [Full Text] [PDF]

				R. C. R. Meex, P. Schrauwen, and M. K. C. Hesselink Modulation of myocellular fat stores: lipid droplet dynamics in health and disease Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2009; 297(4): R913 - R924. [Abstract] [Full Text] [PDF]

				J. M. Sapiro, M. T. Mashek, A. S. Greenberg, and D. G. Mashek Hepatic triacylglycerol hydrolysis regulates peroxisome proliferator-activated receptor {alpha} activity J. Lipid Res., August 1, 2009; 50(8): 1621 - 1629. [Abstract] [Full Text] [PDF]

				V. Bezaire, A. Mairal, C. Ribet, C. Lefort, A. Girousse, J. Jocken, J. Laurencikiene, R. Anesia, A.-M. Rodriguez, M. Ryden, et al. Contribution of Adipose Triglyceride Lipase and Hormone-sensitive Lipase to Lipolysis in hMADS Adipocytes J. Biol. Chem., July 3, 2009; 284(27): 18282 - 18291. [Abstract] [Full Text] [PDF]

				C. Bourdon, S. Hojna, M. Jordan, J. Berube, V. Kren, M. Pravenec, P. Liu, S. Arab, and Z. Pausova Genetic locus on rat chromosome 20 regulates diet-induced adipocyte hypertrophy: a microarray gene expression study Physiol Genomics, June 10, 2009; 38(1): 63 - 72. [Abstract] [Full Text] [PDF]

				S. W. Eastman, M. Yassaee, and P. D. Bieniasz A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover J. Cell Biol., March 23, 2009; 184(6): 881 - 894. [Abstract] [Full Text] [PDF]

				R. Zechner, P. C. Kienesberger, G. Haemmerle, R. Zimmermann, and A. Lass Adipose triglyceride lipase and the lipolytic catabolism of cellular fat stores J. Lipid Res., January 1, 2009; 50(1): 3 - 21. [Abstract] [Full Text] [PDF]

				M. Prentki and S. R. M. Madiraju Glycerolipid Metabolism and Signaling in Health and Disease Endocr. Rev., October 1, 2008; 29(6): 647 - 676. [Abstract] [Full Text] [PDF]

				Y. Imanishi, W. Sun, T. Maeda, A. Maeda, and K. Palczewski Retinyl Ester Homeostasis in the Adipose Differentiation-related Protein-deficient Retina J. Biol. Chem., September 5, 2008; 283(36): 25091 - 25102. [Abstract] [Full Text] [PDF]

				M. Bell, H. Wang, H. Chen, J. C. McLenithan, D.-W. Gong, R.-Z. Yang, D. Yu, S. K. Fried, M. J. Quon, C. Londos, et al. Consequences of Lipid Droplet Coat Protein Downregulation in Liver Cells: Abnormal Lipid Droplet Metabolism and Induction of Insulin Resistance Diabetes, August 1, 2008; 57(8): 2037 - 2045. [Abstract] [Full Text] [PDF]

				J. L. Cocchiaro, Y. Kumar, E. R. Fischer, T. Hackstadt, and R. H. Valdivia From the Cover: Cytoplasmic lipid droplets are translocated into the lumen of the Chlamydia trachomatis parasitophorous vacuole PNAS, July 8, 2008; 105(27): 9379 - 9384. [Abstract] [Full Text] [PDF]

				M. Schweiger, G. Schoiswohl, A. Lass, F. P. W. Radner, G. Haemmerle, R. Malli, W. Graier, I. Cornaciu, M. Oberer, R. Salvayre, et al. The C-terminal Region of Human Adipose Triglyceride Lipase Affects Enzyme Activity and Lipid Droplet Binding J. Biol. Chem., June 20, 2008; 283(25): 17211 - 17220. [Abstract] [Full Text] [PDF]

				P. Keller, J. T. Petrie, P. De Rose, I. Gerin, W. S. Wright, S.-H. Chiang, A. R. Nielsen, C. P. Fischer, B. K. Pedersen, and O. A. MacDougald Fat-specific Protein 27 Regulates Storage of Triacylglycerol J. Biol. Chem., May 23, 2008; 283(21): 14355 - 14365. [Abstract] [Full Text] [PDF]