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Originally published In Press as doi:10.1194/jlr.C600022-JLR200 on January 16, 2007 Originally published In Press as doi:10.1194/jlr.C600022-JLR200 on December 21, 2006

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Journal of Lipid Research, Vol. 48, 513-517, March 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Short Communication

Docosahexaenoic acid and arachidonic acid are fundamental supplements for the induction of neuronal differentiation

Inna Kan*, Eldad Melamed*, Daniel Offen* and Pnina Green1,{dagger}

* Laboratory of Neurosciences, Felsenstein Medical Research Center, Beilinson Campus, Sackler School of Medicine, Tel Aviv University, Petah Tiqwa 49100, Israel
{dagger} Laboratory for the Study of Fatty Acids, Felsenstein Medical Research Center, Beilinson Campus, Sackler School of Medicine, Tel Aviv University, Petah Tiqwa 49100, Israel

Published, JLR Papers in Press, December 21, 2006.

1 To whom correspondence should be addressed. e-mail: pgreen{at}post.tau.ac.il(P.G.)


ABSTRACT

Cell replacement therapy is being investigated for the treatment of neurodegenerative disorders. Adult autologous bone marrow-derived mesenchymal stem cells (MSCs) have been induced to differentiate into neuron-like cells harboring a variety of neuronal markers and transcription factors. Neural tissue characteristically contains high proportions of docosahexaenoic acid (DHA) and arachidonic acid (AA). In this study, evaluation of the fatty acid profile of differentiated neuron-like cells revealed a very low level of DHA, similar to that in MSCs but different from typical neurons. Supplementation of the medium with DHA alone resulted in increased levels of DHA but concomitant low levels of AA. However, supplementation with both DHA and AA yielded a fatty acid profile resembling that of neural tissue. It also resulted in enhanced outgrowth of neurite-like processes, hallmarks of neuronal differentiation. These findings demonstrate the essentiality of DHA and AA supplementation in the process of induced neuronal differentiation and have important implications for the development of cell replacement strategies of neural repair.

Supplementary key words mesenchymal stem cells • neurite growth • cell replacement therapy • polyunsaturated fatty acids

Abbreviations: AA, arachidonic acid; DHA, docosahexaenoic acid; MSC, mesenchymal stem cell


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