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Papers In Press, published online ahead of print March 1, 2007
J. Lipid Res., doi:10.1194/jlr.M600255-JLR200

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Journal of Lipid Research, Vol. 48, 528-542, March 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Importance of the carboxyl terminus of FAT/CD36 for plasma membrane localization and function in long-chain fatty acid uptake

Nicholas S. Eyre^*, Leslie G. Cleland, Narendra N. Tandon and Graham Mayrhofer^1,*,

^* School of Molecular and Biomedical Science, University of Adelaide, Adelaide, South Australia, Australia
Arthritis Research Laboratory, Hanson Institute for Medical Research, Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia
Thrombosis Research Laboratory, Otsuka Maryland Medicinal Laboratories, Rockville, MD

The online version of this article (available at http://www.jlr.org) contains supplementary data in the form of 1 figure.

Published, JLR Papers in Press, December 1, 2006.

¹ To whom correspondence should be addressed. e-mail: graham.mayrhofer{at}adelaide.edu.au

This study investigates the role of the cytoplasmic C terminus of fatty acid translocase (FAT/CD36) in localization of the molecule to the plasma membrane, its insertion into lipid rafts, and its ability to enhance long-chain fatty acid uptake in transfected H4IIE rat hepatoma cells. In these cells, wild-type FAT/CD36 is localized to both lipid raft and nonraft domains of the plasma membrane. Interestingly, a FAT/CD36 truncation mutant lacking the final 10 amino acids of the cytoplasmic C terminus was retained within the cell in detergent-resistant membranes, and unlike wild-type FAT/CD36, it did not enhance oleate uptake. Furthermore, expression of FAT/CD36 in these cells increased the incorporation of oleate into diacylglycerol, a property that was not shared by truncated FAT/CD36. To examine whether the C terminus itself has an intrinsic ability to dictate the plasma membrane localization of FAT/CD36, this region was fused in-frame to enhanced green fluorescent protein (EGFP). This domain was sufficient to attach EGFP to cellular membranes, suggesting an involvement in the intracellular traffic of the molecule. We conclude that the C terminus of FAT/CD36 is required for localization of the receptor to the cell surface and its ability to enhance cellular oleate uptake.

Supplementary key words fatty acid translocase • lipid rafts • caveolae • caveolin-1 • detergent-resistant membranes • oleate • oleic acid • liver

Abbreviations: CT-B, cholera toxin B subunit; DGAT, diacylglycerol acyltransferase; DPBS, Dulbecco's phosphate-buffered saline; DRM, detergent-resistant membrane; EGFP, enhanced green fluorescent protein; FAT, fatty acid translocase; LCFA, long-chain fatty acid; OxLDL, oxidized low density lipoprotein

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