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Originally published In Press as doi:10.1194/jlr.M600421-JLR200 on December 5, 2006

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Journal of Lipid Research, Vol. 48, 683-692, March 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Modulation of the activity and arachidonic acid selectivity of group X secretory phospholipase A2 by sphingolipids

Dev K. Singh and Papasani V. Subbaiah1

Departments of Medicine and Biochemistry & Molecular Genetics, University of Illinois at Chicago, Chicago, IL 60612

Published, JLR Papers in Press, December 5, 2006.

1 To whom correspondence should be addressed. e-mail: psubbaia{at}uic.edu

To investigate the role of sphingomyelin (SM) in the regulation of inflammatory reactions, we studied its effect on the activity and fatty acid specificity of group X secretory phospholipase A2 (sPLA2X). Compared with other phospholipases, recombinant sPLA2X released more arachidonate from HDL. Pretreatment of HDL with sphingomyelinase (SMase) C activated the sPLA2X activity, but the release of arachidonate was stimulated less than that of linoleate. In liposomes containing synthetic phosphatidylcholines (PCs), sPLA2X showed no clear selectivity among the various sn-2 unsaturated fatty acids. However, when SM was incorporated into liposomes at 30 mol%, the enzyme exhibited strong preference for arachidonate, although its overall activity was inhibited. Degradation of liposomal SM by SMase C resulted in sPLA2X activation and loss of its arachidonate preference. Incorporation of ceramide into HDL or PC liposomes activated the enzyme activity, the release of arachidonate being stimulated more than that of linoleate. SM-deficient cells released more arachidonate than normal cells in response to exogenous sPLA2X. SMase pretreatment of normal cells stimulated the release of arachidonate by the exogenous sPLA2X. These results show that SM not only inhibits sPLA2X activity but also contributes to its selectivity for arachidonate, whereas ceramide stimulates the hydrolysis of arachidonate-containing PCs.

Supplementary key words sphingomyelin • ceramide • fatty acid specificity • inflammation

Abbreviations: PC, phosphatidylcholine; SM, sphingomyelin; SMase, sphingomyelinase; sPLA2, secretory phospholipase A2


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