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Papers In Press, published online ahead of print April 1, 2007
J. Lipid Res., doi:10.1194/jlr.M600413-JLR200

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Journal of Lipid Research, Vol. 48, 837-847, April 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Lipidomics reveals that adiposomes store ether lipids and mediate phospholipid traffic^1,,

René Bartz^*, Wen-Hong Li^*, Barney Venables, John K. Zehmer^*, Mary R. Roth, Ruth Welti, Richard G. W. Anderson^*, Pingsheng Liu^1,2,* and Kent D. Chapman^2,

^* Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9039
Center for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX 76203-1277
Kansas Lipidomics Research Center, Division of Biology, Kansas State University, Manhattan, KS 66506

¹ These two groups contributed equally to the project (Group 1: Bartz, Li, Zehmer, Anderson, and Liu; Group 2: Venables, Roth, Welti, and Chapman).

The online version of this article (available at http://www.jlr.org) contains supplemental data in the form of 3 tables and 4 figures.

Published, JLR Papers in Press, January 8, 2007.

² To whom correspondence should be addressed. e-mail: chapman{at}unt.edu (K.D.C.); pingsheng.liu{at}southwestern.edu (P.L.)

Lipid droplets are accumulations of neutral lipids surrounded by a monolayer of phospholipids and associated proteins. Recent proteomic analysis of isolated droplets suggests that they are part of a dynamic organelle system that is involved in membrane traffic as well as packaging and distributing lipids in the cell. To gain a better insight into the function of droplets, we used a combination of mass spectrometry and NMR spectroscopy to characterize the lipid composition of this compartment. In addition to cholesteryl esters and triacylglycerols with mixed fatty acid composition, we found that 10–20% of the neutral lipids were the ether lipid monoalk(en)yl diacylglycerol. Although lipid droplets contain only 1–2% phospholipids by weight, >160 molecular species were identified and quantified. Phosphatidylcholine (PC) was the most abundant class, followed by phosphatidylethanolamine (PE), phosphatidylinositol, and ether-linked phosphatidylcholine (ePC). Relative to total membrane, droplet phospholipids were enriched in lysoPE, lysoPC, and PC but deficient in sphingomyelin, phosphatidylserine, and phosphatidic acid. These results suggest that droplets play a central role in ether lipid metabolism and intracellular lipid traffic.

Supplementary key words lipid droplet • membranes • phospholipid turnover

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