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Journal of Lipid Research, Vol. 48, 848-854, April 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Altered brain lipid composition in cyclooxygenase-2 knockout mouse

Kaizong Ma^*, Robert Langenbach, Stanley I. Rapoport^* and Mireille Basselin^1,*

^* Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892
National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709

Published, JLR Papers in Press, January 3, 2007.

¹ To whom correspondence should be addressed. e-mail: mirvasln{at}mail.nih.gov

Cyclooxygenase (COX)-2 plays an important role in brain arachidonic acid (20:4n-6) metabolism, and its expression is upregulated in animal models of neuroinflammation and excitotoxicity. Our hypothesis was that brain lipid composition would be altered in COX-2 knockout (COX-2^–/–) compared with wild-type (COX-2^+/+) mice, reflecting the important role of COX-2 in brain lipid metabolism. Concentrations of different lipids were measured in high-energy microwaved brain from COX-2^–/– and COX-2^+/+ mice. Compared with the COX-2^+/+ mouse brain, the brain of the COX-2^–/– mouse had a statistically significant 15% increase in phosphatidylserine (PtdSer) and significant 37, 27, and 32% reductions in triacylglycerol and cholesterol concentrations and in the cholesterol-to-phospholipid ratio, respectively. The normalized concentration of palmitic acid (16:0) was increased in PtdSer, as was the brain concentration of unesterified arachidic acid (20:0). A lifetime absence of COX-2 produces multiple changes in brain lipid composition. These changes may be related to reported changes in fatty acid kinetics and in resistance to neuroinflammation and excitotoxicity in the COX-2^–/– mouse.

Supplementary key words fatty acids • cholesterol • phosphatidylserine

Abbreviations: AA, arachidonic acid; CerPCho, sphingomyelin; ChoGpl, choline glycerophospholipid; COX, cyclooxygenase; cPLA₂, cytosolic phospholipase A₂; EtnGpl, ethanolamine glycerophospholipid; FAME, fatty acid methyl ester; PtdCho, phosphatidylcholine; PtdEtn, phosphatidylethanolamine; PtdIns, phosphatidylinositol; PtdSer, phosphatidylserine; sPLA₂, secretory phospholipase A₂

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