HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M600471-JLR200v1 48/5/1069    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Laurencikiene, J. Articles by Rydén, M. Search for Related Content PubMed PubMed Citation Articles by Laurencikiene, J. Articles by Rydén, M. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 48, 1069-1077, May 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

NF-B is important for TNF--induced lipolysis in human adipocytes

Jurga Laurencikiene^1,*, Vanessa van Harmelen^1,*, Elisabet Arvidsson Nordström^*, Andrea Dicker^*, Lennart Blomqvist^*, Erik Näslund, Dominique Langin^,**,, Peter Arner^* and Mikael Rydén^2,*

^* Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden
Department of Surgery, Karolinska Institutet, Danderyd Hospital, 182 88, Stockholm, Sweden
Institut National de la Santé et de la Recherche Médicale U586, Unité de Recherches sur les Obésités, Toulouse, F-31432 France
^** Université Paul Sabatier, Institut Louis Bugnard IFR31, Toulouse, F-31432 France
Centre Hospitalier Universitaire de Toulouse, Biochimie, Institut Fédératif de Biologie de Purpan, Toulouse, F-31059 France

Published, JLR Papers in Press, February 1, 2007.

¹ J. Laurencikiene and V. van Harmelen contributed equally to this work.

² To whom correspondence should be addressed. e-mail: mikael.ryden{at}ki.se

Tumor necrosis factor- (TNF-) promotes lipolysis in mammal adipocytes via the mitogen-activated protein kinase (MAPK) family, resulting in reduced expression/function of perilipin (PLIN). The role of another pivotal intracellular messenger activated by TNF-, nuclear factor-B (NF-B), has not been recognized. We explored the role of NF-B in TNF--induced lipolysis of human fat cells. Primary cultures of human adipocytes were incubated in the presence of a cell-permeable peptide that inhibits NF-B signaling (WP). Incubation with WP, but not with a biologically inactive peptide (MP), abolished the nuclear translocation of NF-B and effectively abrogated TNF--induced lipolysis in a concentration-dependent manner. Western blot analysis demonstrated that although TNF- per se reduced mainly PLIN protein expression, TNF- in the presence of WP resulted in a pronounced combined reduction of both hormone-sensitive lipase (HSL) and PLIN protein. The expression of a set of other lipolytic or adipocyte-specific proteins was not affected. The regulation was presumably at the transcriptional level, because mRNA expression for HSL and PLIN was markedly reduced with TNF- in the presence of NF-B inhibition. This was confirmed in gene reporter assays using human PLIN and HSL promoter constructs. We conclude that in the presence of NF-B inhibition, TNF--mediated lipolysis is reduced, which suggests that NF-B is essential for retained human fat cell lipolysis.

Supplementary key words tumor necrosis factor- • nuclear factor-B • signal • differentiation

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. J. Tisdale Mechanisms of Cancer Cachexia Physiol Rev, April 1, 2009; 89(2): 381 - 410. [Abstract] [Full Text] [PDF]

				L. Zu, J. He, H. Jiang, C. Xu, S. Pu, and G. Xu Bacterial Endotoxin Stimulates Adipose Lipolysis via Toll-Like Receptor 4 and Extracellular Signal-regulated Kinase Pathway J. Biol. Chem., February 27, 2009; 284(9): 5915 - 5926. [Abstract] [Full Text] [PDF]

				J. Laurencikiene, B. M. Stenson, E. Arvidsson Nordstrom, T. Agustsson, D. Langin, B. Isaksson, J. Permert, M. Ryden, and P. Arner Evidence for an Important Role of CIDEA in Human Cancer Cachexia Cancer Res., November 15, 2008; 68(22): 9247 - 9254. [Abstract] [Full Text] [PDF]

				P. Plomgaard, C. P. Fischer, T. Ibfelt, B. K. Pedersen, and G. van Hall Tumor Necrosis Factor-{alpha} Modulates Human in Vivo Lipolysis J. Clin. Endocrinol. Metab., February 1, 2008; 93(2): 543 - 549. [Abstract] [Full Text] [PDF]

				Y. Kawamata, T. Imamura, J. L. Babendure, J.-C. Lu, T. Yoshizaki, and J. M. Olefsky Tumor Necrosis Factor Receptor-1 Can Function through a G{alpha}q/11-beta-Arrestin-1 Signaling Complex J. Biol. Chem., September 28, 2007; 282(39): 28549 - 28556. [Abstract] [Full Text] [PDF]