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Journal of Lipid Research, Vol. 48, 1204-1211, May 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Methods

Reproducibility of stable isotope-labeled tracer measures of VLDL-triglyceride and VLDL-apolipoprotein B-100 kinetics

Faidon Magkos^*,, Bruce W. Patterson^* and Bettina Mittendorfer^1,*

^* Washington University School of Medicine, St. Louis, MO
Department of Nutrition and Dietetics, Harokopio University, Athens, Greece

Published, JLR Papers in Press, February 26, 2007.

¹ To whom correspondence should be addressed. e-mail: mittendb{at}wustl.edu

To gain insight into the mechanisms regulating plasma lipid homeostasis, FFA, VLDL-triglyceride (TG), and VLDL-apolipoprotein B-100 (apoB-100) kinetics are commonly assessed using stable isotope-labeled tracer methods. The reproducibility of these measurements, which is critical for the experimental design, is unknown. Therefore, we investigated the repeatability of plasma FFA, VLDL-TG, and VLDL-apoB-100 kinetics in eight healthy men using stable isotope-labeled tracer techniques. There were no systematic differences in plasma FFA, VLDL-TG, and VLDL-apoB-100 concentrations and kinetics between the two studies. Intraindividual day-to-day variability for various outcome variables ranged from 15% to 25%, and almost all of this was of biological origin. The most robust outcome variables were FFA rate of appearance and hepatic VLDL-TG and VLDL-apoB-100 secretion rates; the least robust were VLDL-TG and VLDL-apoB-100 plasma clearance rates and mean residence times. Overall, physiologically meaningful differences in mean values (i.e., 25–30% in magnitude) can be obtained with a sample size of 6–10 subjects for paired studies and 12–20 subjects per group for cross-sectional studies, assuming a type I error rate of 0.05 and a type II error rate of 0.20 (i.e., 80% power). These findings will be useful for future studies investigating FFA, VLDL-TG, and VLDL-apoB-100 kinetics with the methods described.

Supplementary key words hepatic lipid metabolism • lipoprotein kinetics • repeatability • reliability • very low density lipoprotein

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