|
|
||||||||
Papers In Press, published online ahead of print June 1, 2007
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Journal of Lipid Research, Vol. 48, 1293-1304, June 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology







* Department of Biochemistry, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, South Bend, IN 46617
Department of Biology, Georgia Institute of Technology, Atlanta, GA 30332
** Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607-7061

Research and Development, Hunter Holmes McGuire Veterans Administration Medical Center, Richmond, VA 23249
The online version of this article (available at http://www.jlr.org) contains supplementary data in the form of four figures.
Published, JLR Papers in Press, March 27, 2007.
1 To whom correspondence should be addressed. e-mail: cechalfant{at}vcu.edu
Ceramide kinase (CERK) is a critical mediator of eicosanoid synthesis, and its product, ceramide-1-phosphate (C1P), is required for the production of prostaglandins in response to several inflammatory agonists. In this study, mass spectrometry analysis disclosed that the main forms of C1P in cells were C16:0 C1P and C18:0 C1P, suggesting that CERK uses ceramide transported to the trans-Golgi apparatus by ceramide transport protein (CERT). To this end, downregulation of CERT by RNA interference technology dramatically reduced the levels of newly synthesized C1P (kinase-derived) as well as significantly reduced the total mass levels of C1P in cells. Confocal microscopy, subcellular fractionation, and surface plasmon resonance analysis were used to further localize CERK to the trans-Golgi network, placing the generation of C1P in the proper intracellular location for the recruitment of cytosolic phospholipase A2
. In conclusion, these results demonstrate that CERK localizes to areas of eicosanoid synthesis and uses a ceramide "pool" transported in an active manner via CERT.
Supplementary key words ceramide-1-phosphate prostaglandins phospholipase A2 inflammation arachidonic acid
Abbreviations: AA, arachidonic acid; C1P, ceramide-1-phosphate; CERK, ceramide kinase; CERT, ceramide transport protein; cPLA2, cytosolic phospholipase A2; COX, cyclooxygenase; EEA1, early endosome; EIA, enzyme immuno assay; IL, interleukin; PGE2, prostaglandin E2; siRNA, small interfering RNA; TGN, trans-Golgi network; TOM20, translocon of the outer membrane; SPR, surface plasmon resonance
![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati What's this?
This article has been cited by other articles:
![]() |
F. Revert, I. Ventura, P. Martinez-Martinez, F. Granero-Molto, F. Revert-Ros, J. Macias, and J. Saus Goodpasture Antigen-binding Protein Is a Soluble Exportable Protein That Interacts with Type IV Collagen: IDENTIFICATION OF NOVEL MEMBRANE-BOUND ISOFORMS J. Biol. Chem., October 31, 2008; 283(44): 30246 - 30255. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Boath, C. Graf, E. Lidome, T. Ullrich, P. Nussbaumer, and F. Bornancin Regulation and Traffic of Ceramide 1-Phosphate Produced by Ceramide Kinase: COMPARATIVE ANALYSIS TO GLUCOSYLCERAMIDE AND SPHINGOMYELIN J. Biol. Chem., March 28, 2008; 283(13): 8517 - 8526. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Graf, B. Zemann, P. Rovina, N. Urtz, A. Schanzer, R. Reuschel, D. Mechtcheriakova, M. Muller, E. Fischer, C. Reichel, et al. Neutropenia with Impaired Immune Response to Streptococcus pneumoniae in Ceramide Kinase-Deficient Mice J. Immunol., March 1, 2008; 180(5): 3457 - 3466. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. Subramanian, M. Vora, L. B. Gentile, R. V. Stahelin, and C. E. Chalfant Anionic lipids activate group IVA cytosolic phospholipase A2 via distinct and separate mechanisms J. Lipid Res., December 1, 2007; 48(12): 2701 - 2708. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. P. Herbert, A. F. Odell, S. Ponnambalam, and J. H. Walker The Confluence-dependent Interaction of Cytosolic Phospholipase A2-{alpha} with Annexin A1 Regulates Endothelial Cell Prostaglandin E2 Generation J. Biol. Chem., November 23, 2007; 282(47): 34468 - 34478. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |