J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Originally published In Press as doi:10.1194/jlr.D600042-JLR200 on February 15, 2007

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Journal of Lipid Research, Vol. 48, 1422-1427, June 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Methods

Widespread occurrence of glyceryl ether monooxygenase activity in rat tissues detected by a novel assay

Ernst R. Werner1,*, Albin Hermetter{dagger}, Helmut Prast§, Georg Golderer* and Gabriele Werner-Felmayer*

* Division of Biological Chemistry, Biocenter, Innsbruck Medical University, A-6020 Innsbruck, Austria
{dagger} Institute of Biochemistry, Graz University of Technology, A-8010 Graz, Austria
§ Division of Pharmacology and Toxicology, Institute of Pharmacy, Leopold-Franzens University of Innsbruck, A-6020 Innsbruck Austria

Published, JLR Papers in Press, February 15, 2007.

1 To whom correspondence should be addressed. e-mail: ernst.r.werner{at}i-med.ac.at

An assay was set up for glyceryl ether monooxygenase activity in tissue samples using the novel substrate 1-O-pyrenedecyl-sn-glycerol and high-performance liquid chromatographic analysis of reaction mixtures with fluorescence detection, allowing robust detection of enzymatic activity in microgram amounts of tissue homogenates. The activity partially purified from rat liver strictly depended on the presence of a tetrahydropteridine. Tetrahydrobiopterin-dependent glyceryl ether monooxygenase activity was observed in all rat tissues tested except female heart, with highest activities in liver, intestine, and cerebellum. Activity was not uniformly distributed in brain: it was higher in cerebellum than in striatum or cortex. These data demonstrate that tetrahydrobiopterin-dependent glyceryl ether monooxygenase is found not only in liver and the gastrointestinal tract but also in brain and other organs of the rat and provide an additional goal for tetrahydrobiopterin biosynthesis in these organs.

Supplementary key words tetrahydrobiopterin • pteridine • ether lipid • alkylglycerol • chemical synthesis • activity assay


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Copyright © 2007 by the American Society for Biochemistry and Molecular Biology.