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Journal of Lipid Research, Vol. 48, 1772-1780, August 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology
Department of Laboratory Medicine, Division of Clinical Chemistry, Lund University, University Hospital Malmö, SE-20502 Malmö, Sweden
Published, JLR Papers in Press, May 25, 2007.
1 To whom correspondence should be addressed. e-mail: bj%F6rn.dahlb%E4ck{at}med.lu.se
Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa protein belonging to the lipocalin protein superfamily. Although predominantly associated with high density lipoprotein, apoM is found in all major lipoprotein classes. To facilitate clinical studies of apoM, we have developed a sandwich ELISA for the measurement of apoM in human plasma. This method has been used to investigate normal apoM variation and to establish reference values for healthy individuals through the measurement of 598 samples from the Nordic Reference Interval Project Bio-bank and Database (NOBIDA) biobank. For women 18–49 years old, the reference interval for apoM was 0.58–1.18 µmol/l, whereas for women 50+ years and for men, the reference range was 0.61–1.30 µmol/l. Correlation studies of apoM with 26 common clinical chemical analytes from the NOBIDA database revealed a marked positive correlation with plasma total cholesterol (r = 0.52) and LDL and HDL cholesterol (r = 0.43 and 0.36, respectively). There was no statistically significant correlation with HDL/total cholesterol ratio or body mass index. In conclusion, a sandwich ELISA for the measurement of apoM in human plasma shows that apoM concentration is strongly correlated to total cholesterol in healthy individuals.
Supplementary key words high density lipoprotein • low density lipoprotein • reference interval • reference range • biobank • Nordic Reference Interval Project Bio-bank and Database • Nordic Reference Interval Project • lipocalin • enzyme-linked immunosorbent assay
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