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Originally published In Press as doi:10.1194/jlr.M700152-JLR200 on May 22, 2007

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Journal of Lipid Research, Vol. 48, 1825-1831, August 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Structural elucidation of a novel phosphoglycolipid isolated from six species of Halomonas

Assunta Giordano1, Filomena M. Vella, Ida Romano and Agata Gambacorta

Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, 34 80078 Pozzuoli, Italy

Published, JLR Papers in Press, May 22, 2007.

1 To whom correspondence should be addressed. e-mail: agiordano{at}icmib.na.cnr.it

The structure of a new phosphoglycolipid from the halophilic Gram-negative bacteria Halomonas elongata ATCC 33173T, Halomonas eurihalina ATCC 49336T, Halomonas almeriensis CECT 7050T, strain Sharm (AM238662), Halomonas halophila DSM 4770T, and Halomonas salina ATCC 49509T was elucidated by NMR and mass spectroscopy studies. In all of the species examined, the polar lipid composition consisted of 1,2-diacylglycero-3-phosphorylethanolamine, 1,2-diacylglycero-3-phosphoryl-glycerol, bisphosphatidyl glycerol, and the new phosphoglycolipid PGL1. The structure of PGL1 was established to be (2-({alpha}-D-glucopyranosyloxy)-3-hydroxy-propyl)-phosphatidyl diacylglycerol. C16:0;C18:1 and C16:0;C19:cyclopropane are the most abundant acyl chains linked to the phosphatidylglycerol moiety of each isolated PGL1. All of the species presenting the lipid PGL1 belong to Halomonas rRNA group 1, suggesting that the new phosphoglycolipid could be a chemotaxonomic marker of this phylogenetic group.

Supplementary key words Halomonas elongata ATCC 33173T • Halomonas eurihalina ATCC 49336T • Halomonas almeriensis CECT 7050T • strain Sharm (AM238662) • Halomonas halophila DSM 4770T • Halomonas salina ATCC 49509T • nuclear magnetic resonance spectroscopy • tandem mass spectrometry

Abbreviations: CD, circular dichroism; DPG, bisphosphatidyl glycerol; DQF-COSY, double quantum-filtered correlation spectroscopy; FAME, fatty acid methyl ester; HMBC, heteronuclear multiple bond coherence; HSQC, heteronuclear single quantum coherence; PEA, phosphatidylethanolamine; PG, phosphatidylglycerol


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