J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Originally published In Press as doi:10.1194/jlr.M700131-JLR200 on May 11, 2007

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Journal of Lipid Research, Vol. 48, 1846-1856, August 2007
Copyright © 2007 by American Society for Biochemistry and Molecular Biology

Direct evidence of lipid translocation between adipocytes and prostate cancer cells with imaging FTIR microspectroscopy

Ehsan Gazi1,*, Peter Gardner{dagger}, Nicholas P. Lockyer{dagger}, Claire A. Hart*, Michael D. Brown* and Noel W. Clarke*,§,**

* ProMPT Genito Urinary Cancer Research Group, Paterson Institute for Cancer Research, The University of Manchester, Manchester, UK
{dagger} School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, UK
§ Department of Urology, Hope Hospital, NHS Trust, Manchester, UK
** Department of Urology, Christie Hospital, NHS Trust, Manchester, UK

Published, JLR Papers in Press, May 11, 2007.

1 To whom correspondence should be addressed. e-mail: EGazi{at}picr.man.ac.uk

Various epidemiological studies show a positive correlation between high intake of dietary FAs and metastatic prostate cancer (CaP). Moreover, CaP metastasizes to the bone marrow, which harbors a rich source of lipids stored within adipocytes. Here, we use Fourier transform infrared (FTIR) microspectroscopy to study adipocyte biochemistry and to demonstrate that PC-3 cells uptake isotopically labeled FA [deuterated palmitic acid (D31-PA)] from an adipocyte. Using this vibrational spectroscopic technique, we detected subcellular locations in a single adipocyte enriched with D31-PA using the {upsilon}as+s(C-D)2+3 (D31-PA): {upsilon}as+s(C-H)2+3 (lipid hydrocarbon) signal. In addition, larger adipocytes were found to consist of a higher percentage of D31-PA of the total lipid found within the adipocyte. Following background subtraction, the {upsilon}as(C-D)2+3 signal illuminated starved PC-3 cells cocultured with D31-PA-loaded adipocytes, indicating translocation of the labeled FA. This study demonstrates lipid-specific translocation between adipocytes and tumor cells and the use of FTIR microspectroscopy to characterize various biomolecular features of a single adipocyte without the requirement for cell isolation and lipid extraction.

Supplementary key words tumor • fatty acids • Fourier transform infrared spectroscopy • deuterated palmitic acid

Abbreviations: BMS, bone marrow stroma; CaP, prostate cancer; CPD, critical-point drying; D31-PA, deuterated palmitic acid; FFA, free FA; FTIR, Fourier transform infrared; IL-6, interleukin-6; IR, infrared; MSC, mesenchymal stem cell; PF, paraformaldehyde; SR, synchrotron; TAG, triacylglyceride


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