J. Lipid Res. Acyl Labeled PIP's available August 1, 2008
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Originally published In Press as doi:10.1194/jlr.M700355-JLR200 on October 22, 2007

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Journal of Lipid Research, Vol. 49, 136-146, January 2008
Copyright © 2008 by American Society for Biochemistry and Molecular Biology

Scavenger receptor BI facilitates the metabolism of VLDL lipoproteins in vivo

Miranda Van Eck1, Menno Hoekstra, Ruud Out, I. Sophie T. Bos, J. Kar Kruijt, Reeni B. Hildebrand and Theo J. C. Van Berkel

Divison of Biopharmaceutics, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands

Published, JLR Papers in Press, October 22, 2007.

1 To whom correspondence should be addressed. e-mail: m.eck{at}LACDR.LeidenUniv.nl

Scavenger receptor class B type I (SR-BI) functions as an HDL receptor that promotes the selective uptake of cholesteryl esters (CEs). The physiological role of SR-BI in VLDL metabolism, however, is largely unknown. SR-BI deficiency resulted in elevated VLDL cholesterol levels, both on chow diet and upon challenge with high-cholesterol diets. To specifically elucidate the role of SR-BI in VLDL metabolism, the plasma clearance and hepatic uptake of 125I-β-VLDL were studied in SR-BI+/+ and SR-BI–/– mice. At 20 min after injection, 66 ± 2% of the injected dose was taken up by the liver in SR-BI+/+ mice, as compared with only 22 ± 4% (P = 0.0007) in SR-BI–/– mice. In vitro studies established that the Bmax of 125I-β-VLDL binding was reduced from 469 ± 30 ng/mg in SR-BI+/+ hepatocytes to 305 ± 20 ng/mg (P = 0.01) in SR-BI–/– hepatocytes. Both in vivo and in vitro, limited to no selective uptake of CEs from β-VLDL was found. Interestingly, HDL effectively competed for the association of β-VLDL in the presence as well as in the absence of SR-BI, indicating a second common recognition site. In conclusion, SR-BI plays an important physiological role in the metabolism of VLDL (remnants).

Supplementary key words liver • hepatocytes • gene expression • mouse model


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