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Originally published In Press as doi:10.1194/jlr.M700421-JLR200 on November 17, 2007

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Journal of Lipid Research, Vol. 49, 438-454, February 2008
Copyright © 2008 by American Society for Biochemistry and Molecular Biology

Lipid mapping in human dystrophic muscle by cluster-time-of-flight secondary ion mass spectrometry imaging

Nora Tahallah*, Alain Brunelle*, Sabine De La Porte{dagger} and Olivier Laprévote1,*

* Laboratoire de Spectrométrie de Masse, Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2301, F91198 Gif sur Yvette Cedex, France
{dagger} Laboratoire de Neurobiologie Cellulaire et Moléculaire, Institut de Neurobiologie Alfred Fessard, Centre National de la Recherche Scientifique, FRC2118, Unité Propre de Recherche 9040, F91198 Gif sur Yvette Cedex, France

Published, JLR Papers in Press, November 17, 2007.

1 To whom correspondence should be addressed. e-mail: olivier.laprevote{at}icsn.cnrs-gif.fr

Human striated muscle samples, from male control and Duchenne muscular dystrophy-affected children, were subjected to cluster-time-of-flight secondary ion mass spectrometry (cluster-ToF-SIMS) imaging using a 25 keV Bi3+ liquid metal ion gun under static SIMS conditions. Spectra and ion density maps, or secondary ion images, were acquired in both positive and negative ion mode over several areas of 500 x 500 µm2 (image resolution, 256 x 256 pixels). Characteristic distributions of various lipids were observed. Vitamin E and phosphatidylinositols were found to concentrate within the cells, whereas intact phosphocholines accumulated over the most damaged areas of the dystrophic muscles, together with cholesterol and sphingomyelin species. Fatty acyl chain composition varied depending on the region, allowing estimation of the local damage extent.

Supplementary key words Duchenne muscular dystrophy • human skeletal muscle degeneration • lipidomics • time-of-flight secondary ion mass spectrometry • oxidative stress • regeneration


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