J. Lipid Res.
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Originally published In Press as doi:10.1194/jlr.M700543-JLR200 on February 8, 2008

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Journal of Lipid Research, Vol. 49, 1077-1089, May 2008
Copyright © 2008 by American Society for Biochemistry and Molecular Biology

A fluorescent sphingolipid binding domain peptide probe interacts with sphingolipids and cholesterol-dependent raft domainsboxs

Sarita Hebbar*, Esther Lee*, Manoj Manna*,{dagger}, Steffen Steinert1,*, Goparaju Sravan Kumar2,§, Markus Wenk§, Thorsten Wohland{dagger} and Rachel Kraut3,*

* Institute of Bioengineering and Nanotechnology, The Nanos, Singapore 138669
{dagger} Department of Chemistry, National University of Singapore, Singapore
§ Department of Biochemistry, National University of Singapore, Singapore

boxs The online version of this article (available at http://www.jlr.org) contains supplementary data in the form of four figures.

Published, JLR Papers in Press, February 8, 2008.

1 Present address of S. Steinert: Physikalisches Institut, Technische Universitaet Stuttgart, Pfaffenwaldring 5, D-70550 Stuttgart, Germany.

2 Present address of G. S. Kumar: Division of Biochemistry, Department of Molecular and Cellular Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.

3 To whom correspondence should be addressed. e-mail: rkraut{at}ibn.a-star.edu.sg

We have designed a tagged probe [sphingolipid binding domain (SBD)] to facilitate the tracking of intracellular movements of sphingolipids in living neuronal cells. SBD is a small peptide consisting of the SBD of the amyloid precursor protein. It can be conjugated to a fluorophore of choice and exogenously applied to cells, thus allowing for in vivo imaging. Here, we present evidence to describe the characteristics of the SBD association with the plasma membrane. Our experiments demonstrate that SBD binds to isolated raft fractions from human neuroblastomas and insect neuronal cells. In protein-lipid overlay experiments, SBD interacts with a subset of glycosphingolipids and sphingomyelin, consistent with its raft association in neurons. We also provide evidence that SBD is taken up by neuronal cells in a cholesterol- and sphingolipid-dependent manner via detergent-resistant microdomains. Furthermore, using fluorescence correlation spectroscopy to assay the mobility of SBD in live cells, we show that SBD's behavior at the plasma membrane is similar to that of the previously described raft marker cholera toxin B, displaying both a fast and a slow component. Our data suggest that fluorescently tagged SBD can be used to investigate the dynamic nature of glycosphingolipid-rich detergent-resistant microdomains that are cholesterol-dependent.

Supplementary key words fluorescent probe • lipid rafts • detergent-resistant microdomains • amyloid β peptide • fluorescence correlation spectroscopy

Abbreviations: Aβ, amyloid β peptide; c6, cell line DL-DMBG2-c6; CtxB, cholera toxin B; DiI, dialkyl-indocarbocyanine; DRM, detergent-resistant membrane; FB1, fumonisin B1; FCS, fluorescence correlation spectroscopy; GFP, green fluorescent protein; HFIP, 1,1,1,3,3,3-hexafluoro-2-propanol; MβCD, methyl-β-cyclodextrin; OG, Oregon green; SBD, sphingolipid binding domain; TMR, tetramethyl rhodamine


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