J. Lipid Res.
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Originally published In Press as doi:10.1194/jlr.D800002-JLR200 on February 13, 2008

Papers In Press, published online ahead of print May 1, 2008
J. Lipid Res., doi:10.1194/jlr.D800002-JLR200
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Journal of Lipid Research, Vol. 49, 1126-1129, May 2008
Copyright © 2008 by American Society for Biochemistry and Molecular Biology

Methods

A rapid, microplate SNP genotype assay for the leptinob alleleboxs

Angie T. Oler and Alan D. Attie1

Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706

boxs The online version of this article (available at http://www.jlr.org) contains supplementary data.

Published, JLR Papers in Press, February 13, 2008.

1 To whom correspondence should be addressed. e-mail: attie{at}biochem.wisc.edu

A nonsense mutation in the mouse leptin gene causes genetic obesity. As a result of extensive research in the field of obesity, the use of leptinob mice is widespread. This mutation renders mice sterile, creating the need to breed heterozygous mice. For this reason, leptinob genotyping is necessary. To date, gel-based assays have been used for genotyping. Using the Invader Plus® assay for single nucleotide polymorphism (SNP) detection, we have developed a gel-free microplate SNP assay for genotyping leptinwt and leptinob alleles.

Supplementary key words obesity • obese • single nucleotide polymorphism

Abbreviations: FAM, fluorescein; FRET, fluorescence resonant energy transfer; RFU, relative fluorescence units; SNP, single nucleotide polymorphism


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Copyright © 2008 by the American Society for Biochemistry and Molecular Biology.