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J. Lipid Res.
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Originally published In Press as doi:10.1194/jlr.D800013-JLR200 on April 13, 2008

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Journal of Lipid Research, Vol. 49, 1588-1594, July 2008
Copyright © 2008 by American Society for Biochemistry and Molecular Biology

Methods

Pancreatic contamination of mesenteric adipose tissue samples can be avoided by adjusted dissection procedures

Robert Caesar1 and Christian A. Drevon

Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway

This work was supported by the European Nutrigenomics Organization (NuGO, Grant CT-2004–505944), the Throne-Holst Foundation for Nutrition Research, Freia Chocolade Fabriks Medicinske Fond, and Anders Jahre's Foundation.

Published, JLR Papers in Press, July 1, 2008.

1 To whom correspondence should be addressed. e-mail: l.e.r.caesar{at}medisin.uio.no

Mesenteric adipose tissue, located in the mesenterium of the intestines, is believed to play a central role in the development of obesity-related diseases. We have found that mesenteric fat samples harvested from rodents are frequently of poor quality, exhibiting partly degraded RNA. To investigate the background for this observation, we screened adipose tissue samples from two independent studies on rodents for markers of different tissues and cell types. We found that mesenteric adipose tissue samples of low quality are "contaminated" by pancreatic tissue. To locate the affected area, we dissected the mesenteric fat depots from 14 mice and measured abundance of pancreas-specific gene expression and amylase activity. As expected, we observed that the proximal section of the mesenterium, located near the pancreas, expressed pancreatic markers, whereas the distal sections did not. Approximately one-third of the mesenteric adipose tissue depots contained pancreatic tissue. Because the boundary between pancreas and mesenteric fat cannot be easily distinguished during dissection, we conclude that investigators should routinely exclude the proximal section of the mesenteric adipose tissue depot to avoid pancreatic contamination.

Supplementary key words visceral • rodent • RNA integrity • obesity • abdominal • metabolic syndrome • yield • mouse • rat

Abbreviations: RIN, RNA integrity number; TTA, tetradecylthioacetic acid


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