J. Lipid Res. Did you know there is a large type edition? Click here.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1194/jlr.M800117-JLR200 on May 9, 2008

Papers In Press, published online ahead of print September 1, 2008
J. Lipid Res., doi:10.1194/jlr.M800117-JLR200
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M800117-JLR200v1
49/9/1963    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by DeMar, J. C.
Right arrow Articles by Salem, N.
PubMed
Right arrow PubMed Citation
Right arrow Articles by DeMar, J. C., Jr.
Right arrow Articles by Salem, N., Jr.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 49, 1963-1980, September 2008
Copyright © 2008 by American Society for Biochemistry and Molecular Biology

Effect of dietary docosahexaenoic acid on biosynthesis of docosahexaenoic acid from alpha-linolenic acid in young ratsboxs

James C. DeMar, Jr.*, Carmine DiMartino*, Adam W. Baca*, William Lefkowitz{dagger} and Norman Salem, Jr.1,*

* Section of Nutritional Neurosciences, Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892
{dagger} Uniformed Services University of the Health Sciences, Bethesda, MD 20814

boxs The online version of this article (available at http://www.jlr.org) contains supplementary data in the form of nine tables.

Published, JLR Papers in Press, May 9, 2008.

This project was supported by the Intramural Research Program of the National Institute on Alcohol Abuse and Alcoholism.

1 To whom correspondence should be addressed: e-mail: nsalem{at}niaaa.nih.gov or nsalem{at}martek.com

Docosahexaenoic acid (DHA), a crucial nervous system n-3 PUFA, may be obtained in the diet or synthesized in vivo from dietary {alpha}-linolenic acid (LNA). We addressed whether DHA synthesis is regulated by the availability of dietary DHA in artificially reared rat pups, during p8 to p28 development. Over 20 days, one group of rat pups was continuously fed deuterium-labeled LNA (d5-LNA) and no other n-3 PUFA (d5-LNA diet), and a second group of rat pups was fed a d5-LNA diet with unlabeled DHA (d5-LNA + DHA diet). The rat pups were then euthanized, and the total amount of deuterium-labeled docosahexaenoic acid (d5-DHA) (synthesized DHA) as well as other n-3 fatty acids present in various body tissues, was quantified. In the d5-LNA + DHA group, the presence of dietary DHA led to a marked decrease (3- to 5-fold) in the total amount of d5-DHA that accumulated in all tissues that we examined, except in adipose. Overall, DHA accretion from d5-DHA was generally diminished by availability of dietary preformed DHA, inasmuch as this was found to be the predominant source of tissue DHA. When preformed DHA was unavailable, d5-DHA and unlabeled DHA were preferentially accreted in some tissues along with a net loss of unlabeled DHA from other organs.

Supplementary key words essential fatty acids • lipid metabolism • early development • infant formula composition

Abbreviations: AA, arachidonic acid (20:4n-6); BHT, butylated hydroxytoluene; DHA, docosahexaenoic acid (22:6n-3); d5-DHA, deuterium-labeled docosahexaenoic acid; DPA, docosapentaenoic acid (22:5n-3); d5-DPA, deuterium-labeled docosapentaenoic acid; EPA, eicosapentaenoic acid (20:5n-3); d5-EPA, deuterium-labeled eicosapentaenoic acid; FAME, fatty acid methyl ester; LA, linoleic acid (18:2n-6); LNA, {alpha}-linolenic acid (18:3n-3); d5-LNA, deuterium-labeled {alpha}-linolenic acid; RBC, red blood cell


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2008 by the American Society for Biochemistry and Molecular Biology.