J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1194/jlr.M800200-JLR200 on May 23, 2008

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M800200-JLR200v1
49/9/1990    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Farias, S. E.
Right arrow Articles by Murphy, R. C.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Farias, S. E.
Right arrow Articles by Murphy, R. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 49, 1990-2000, September 2008
Copyright © 2008 by American Society for Biochemistry and Molecular Biology

Formation of eicosanoids, E2/D2 isoprostanes, and docosanoids following decapitation-induced ischemia, measured in high-energy-microwaved rat brain*

Santiago E. Farias*, Mireille Basselin{dagger}, Lisa Chang{dagger}, Kim A. Heidenreich*, Stanley I. Rapoport{dagger} and Robert C. Murphy1,*

* Department of Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045
{dagger} Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892

* This work was supported in part by the Heart, Lung and Blood Institute, National Institutes of Health, Grant HL-025785, and a grant from General Medical Sciences (GM-069338) (Lipid Maps), as well as by the Intramural Research Program of the National Institute on Aging, National Institutes of Health. No author has a conflict of interest or financial interest with regard to this work.

Published, JLR Papers in Press, May 23, 2008.

1 To whom correspondence should be addressed. e-mail: Robert.Murphy{at}uchsc.edu

Inflammatory lipid mediators derived from arachidonic acid (AA) and docosahexaenoic acid (DHA) modify the pathophysiology of brain ischemia. The goal of this work was to investigate the formation of eicosanoids and docosanoids generated from AA and DHA, respectively, during no-flow cerebral ischemia. Rats were subjected to head-focused microwave irradiation 5 min following decapitation (complete ischemia) or prior to decapitation (controls). Brain lipids were extracted and analyzed by reverse-phase liquid chromatography-tandem mass spectrometry. After complete ischemia, brain AA, DHA, and docosapentaenoic acid concentrations increased 18-, 5- and 4-fold compared with controls, respectively. Prostaglandin E2 (PGE2) and PGD2 could not be detected in control microwaved rat brain, suggesting little endogenous PGE2/D2 production in the brain in the absence of experimental manipulation. Concentrations of thromboxane B2, E2/D2-isoprostanes, 5-hydroxyeicosatetraenoic acid (5-HETE), 5-oxo-eicosatetraenoic acid, and 12-HETE were significantly elevated in ischemic brains. In addition, DHA products such as mono-, di- and trihydroxy-DHA were detected in control and ischemic brains. Monohydroxy-DHA, identified as 17-hydroxy-DHA and thought to be the immediate precursor of neuroprotectin D1, was 6.5-fold higher in ischemic than in control brain. The present study demonstrated increased formation of eicosanoids, E2/D2-IsoPs, and docosanoids following cerebral ischemia. A balance of these lipid mediators may mediate immediate events of ischemic injury and recovery.

Supplementary key words arachidonic • docosahexaenoic • eicosanoids • docosanoids • isoprostanes • brain ischemia

Abbreviations: AA, arachidonic acid; COX, cyclooxygenase; DHA, docosahexaenoic acid; DPA, docosapentaenoic acid; 5-oxo-ETE, 5-oxo-eicosatetraenoic acid; HETE, hydroxyeicosatetraenoic acid; IsoP, isoprostane; LC/MS/MS, liquid chromatography-tandem mass spectrometry; LOX, lipoxygenase; LT, leukotriene; MRM, multiple reaction monitoring; NPD1, neuroprotectin D1; PGD2, prostaglandin D2; RT, retention time; TXB2, thromboxane B2


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2008 by the American Society for Biochemistry and Molecular Biology.