J. Lipid Res.  Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 5, 20-27, January 1964
Copyright © 1964 by Lipid Research, Inc.

Quantitative Analysis of Fatty Acids by Gas-Liquid Chromatography

E. C. Horning 1, E. H. Ahrens Jr. 2, S. R. Lipsky 3, F. H. Mattson 4, J. F. Mead 5, D. A. Turner 6, and W. H. Goldwater 7

1 Baylor University College of Medicine, Houston, Texas
2 The Rockefeller Institute, New York, New York
3 Yale University School of Medicine, New Haven, Connecticut
4 Procter and Gamble Co., Cincinnati, Ohio
5 School of Medicine, University of California at Los Angeles, Los Angeles, California
6 Sinai Hospital, Baltimore, Maryland
7 National Heart Institute, National Institutes of Health, Bethesda 14, Maryland

The fatty acid methyl ester mixtures obtainable from the National Heart Institute, Bethesda, Md., U.S.A., as reference standards for gas-liquid chromatography (GLC) have been examined in a number of laboratories. The results of experience with the mixtures are summarized and detailed recommendations are given of their use in assessing the accuracy of GLC equipment and procedures. The effects of several features of instrument design and operating conditions are discussed, and the recognition and correction of commonly encountered defects are described.

Submitted on October 8, 1963
Accepted on October 16, 1963


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