Journal of Lipid Research, Vol. 5, 75-80, January 1964
Copyright © 1964 by Lipid Research, Inc.
The structure of plasmalogens: VII. Analysis of mammalian liver lipids and the interference of vitamin A in the analysis
German Camejo , Maurice M. Rapport , and Gene A. Morrill
Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, New York 61, New York
Low values of the molar ratio of 
,ßbeta;-unsaturated ether to aldehydogenic lipid found with lipid extracts of mouse and rat liver are shown to result solely from the presence in these extracts of vitamin A rather than of long-chain aldehydes or mixed acetals. Vitamin A interferes in the spectrophotometric determination of specific iodination of ,ßbeta;-unsaturated ethers because it absorbs some light at 355 mµ. The correction is made simply by using an appropriate sample blank. Vitamin A interferes much more seriously with the estimation of aldehydogenic lipids as p-nitrophenylhydrazones because of its transformation under the reaction conditions to anhydro vitamin A. The correction for this interference may be made by independent estimation of the vitamin A content (Carr-Price reaction), determining a standard curve for vitamin A subjected to the p-nitrophenylhydrazone procedure, and subtracting the appropriate value.
The vitamin A content of liver is dependent on the species of animal as well as on diet. In the animals studied, the average level of interference of vitamin A in estimations of liver plasmalogen was 75% for the mouse, 50% for the rat, 8% for the rabbit, and negligible for guinea pig.
Submitted on July 3, 1963
Accepted on September 30, 1963
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