Journal of Lipid Research, Vol. 5, 98-102, January 1964
Copyright © 1964 by Lipid Research, Inc.

Structures of the ester-linked mono- and diunsaturated fatty acids of pig brain

Yasuo Kishimoto and Norman S. Radin

Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan

The structural characterization of mono- and diunsaturated fatty acids present in ester form in pig brain is described. The acids were isolated by mild saponification of total lipids and esterified. The esters were then separated using column, thin-layer, and gas-liquid chromatography, and the positions of the double bonds were determined by oxidative ozonolysis. Forty-seven acids were found, 25 of which have not been previously reported to occur in natural sources. These include the even- and odd-numbered monoenoic acids and the even-numbered dienoic acids 16 to 22 carbon atoms long. Each acid proved to consist of three to six positional isomers. Judging by the positions of the double bonds, the longer monoenoic acids appear to be formed by chain elongation with C₂ units from shorter unsaturated acids: 16:1⁹ (palmitoleate), 16:1⁶, 17:1⁹, 18:1⁹ (oleate), and 19:1⁹. The dienoic acids appear to be formed from linoleate, palmitoleate, oleate, and 16:1⁶.

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