HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Oliveira, M. M. Articles by Vaughan, M. Search for Related Content PubMed Articles by Oliveira, M. M. Articles by Vaughan, M. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 5, 156-162, April 1964
Copyright © 1964 by Lipid Research, Inc.

Incorporation of fatty acids into phospholipids of erythrocyte membranes

Mecia M. Oliveira and Martha Vaughan

Laboratory of Metabolism, National Heart Institute, National Institutes of Health, Bethesda, Maryland

Incorporation of C¹⁴-labeled fatty acids into phospholipids by washed ghosts, prepared from human erythrocytes, required the addition of ATP and was markedly stimulated by MgCl₂ and coenzyme A. Labeled palmitic acid from 1-C¹⁴-palmitoyl CoA was incorporated in the absence of added cofactors. The extent of incorporation of linoleic acid was greater than that of oleic, palmitic, or stearic acids. Of the radioactive fatty acid taken up into phospholipids 80-90% was found in the lecithin fraction. Degradation of the lecithin with Crotalus adamanteus venom established that the labeled fatty acid was located entirely in the ßbeta;-position. When whole blood was incubated with labeled fatty acid it was also found that most of the radioactivity incorporated into the ghost phospholipids was in the ßbeta;-position of lecithin.

Rat erythrocyte ghosts incorporated C¹⁴-linoleic acid into phospholipids at about the same rate as did human erythrocyte ghosts. Sheep erythrocyte ghosts (which contain little lecithin) incorporated very little fatty acid into phospholipids, and none into lecithin.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E. Soupene, H. Fyrst, and F. A. Kuypers Mammalian acyl-CoA:lysophosphatidylcholine acyltransferase enzymes PNAS, January 8, 2008; 105(1): 88 - 93. [Abstract] [Full Text] [PDF]