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Journal of Lipid Research, Vol. 5, 163-168, April 1964
Copyright © 1964 by Lipid Research, Inc.

Incorporation of C14-labeled acetate into lipids by macrophages in vitro

A. J. Day and N. H. Fidge

Department of Human Physiology and Pharmacology, University of Adelaide, South Australia

The incorporation of C14-labeled acetate into lipids by macrophages was investigated using rabbit peritoneal macrophages incubated in vitro. Incorporation of acetate into lipid was shown to occur, the specific activity of the cholesterol labeled being 10-15 times that of the fatty acid. Fractionation of the labeled lipid on silicic acid columns demonstrated the incorporation of the C14-labeled acetate into cholesterol, cholesterol ester, triglyceride, mono- and diglyceride, and phospholipid. The importance of lipid synthesis by macrophages was indicated by the conversion to lipid of 27% of the acetate taken up by the macrophages. Of this 70-80% was incorporated into the nonsaponifiable fraction, 20-30% into fatty acid and about 1% into lipid glycerol.

Submitted on October 1, 1963
Accepted on December 17, 1963


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