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Journal of Lipid Research, Vol. 5, 184-187, April 1964
Copyright © 1964 by Lipid Research, Inc.

Enzymological determination of free carnitine concentrations in rat tissues

Norman R. Marquis and Irving B. Fritz

Department of Physiology, University of Michigan, Ann Arbor, Michigan

Purified carnitine acetyltransferase was employed to catalyze the reaction:

Carnitine + acetyl CoA acetylcarnitine + CoA.

In the presence of a known excess of acetyl CoA, free CoA released was determined simultaneously in a coupled spectrophotometric assay. CoA formed was shown to be directly related to carnitine initially present. Rat tissue extracts were analyzed using this method, and µmoles of (-)-carnitine per gram dry weight were found to be 4.87 for heart; 3.14 for brown adipose tissue; 2.68 for skeletal muscle; 1.74 for testes; 1.26 for kidney; 0.55 for brian; and 0.051 for serum. The specificity and accuracy of the determinations are described.

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