HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ho, R. J. Articles by Meng, H. C. Search for Related Content PubMed Articles by Ho, R. J. Articles by Meng, H. C. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 5, 203-209, April 1964
Copyright © 1964 by Lipid Research, Inc.

A technique for the cannulation and perfusion of isolated rat epididymal fat pad

R. J. Ho and H. C. Meng

Department of Physiology, Vanderbilt University School of Medicine, Nashville, Tennessee

A method for study of the rat epididymal fat pad in vitro is described, in which perfusion via the internal spermatic artery is employed. The time interval between the stoppage of the blood flow and the establishment of the perfusion is less than 30 sec. Observations of perfusion pressure, flow rate, tissue edema, and oxygen consumption indicate that this system can be used to study the metabolism of adipose tissue. The sensitivity of the method with respect to free fatty acid release stimulated by epinephrine and ACTH is greater than that of incubated tissue, probably because of the increase in surface area of the fat cells exposed to the medium

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. Consol, A. Moles, D. Ricart-Jane, and M. Llobera An in situ perfusion protocol of rat epididymal adipose tissue useful in metabolic studies J. Lipid Res., August 1, 2005; 46(8): 1803 - 1808. [Abstract] [Full Text] [PDF]