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Journal of Lipid Research, Vol. 5, 263-265, April 1964
Copyright © 1964 by Lipid Research, Inc.

Improved determination of glycerol and fatty acids in glycerides and ethanolamine phosphatides by gas-liquid chromatography

K. S. Holla , Lloyd A. Horrocks , and David G. Cornwell

Department of Physiological Chemistry, The Oh o State University, Columbus, Ohio, and Laboratory of Neurochemistry, Cleveland Psychiatric Institute, Cleveland, Ohio

The fatty acid and glycerol content of glycerides were estimated by a simplified hydrogenolysis-acetylation procedure and GLC. When fatty acids interfered in the analysis, glycerol alone was estimated by saponification-acetylation and GLC. Preliminary acetolysis was necessary for the estimation of glycerol in ethanolamine phosphatides by these methods.

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