J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 5, 270-272, April 1964
Copyright © 1964 by Lipid Research, Inc.

A rapid chemical method for quantification of lipids separated by thin-layer chromatography

J. S. Amenta

Armed Forces Institute of Pathology, Washington, D. C.

Lipids, separated by thin-layer chromatography, are oxidized in an acid dichromate solution. The reduction in absorbance at 350 mµ is proportional to the amount of lipid. One reagent suffices to quantify all lipid classes to a lower limit of 15 µg of lipid.

Submitted on September 5, 1963
Accepted on November 26, 1963


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