J. Lipid Res.
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Journal of Lipid Research, Vol. 5, 273-274, April 1964
Copyright © 1964 by Lipid Research, Inc.

A method for rapid microsynthesis of radioactive cholesterol esters

Karoly G. Pinter , James G. Hamilton , and James E. Muldrey

Nutrition and Metabolism Laboratory of the Department of Medicine and the Department of Biochemistry, Tulane University School of Medicine, New Orleans, Louisiana

A simple method for synthesizing esters of labeled cholesterol of high specific activity is described. The formation of acyl chlorides takes 10 min and they are reacted with labeled cholesterol to form the cholesterol esters in another 5 min. Yields were 30-70% and radiopurities of 90-99.5% were achieved.

Submitted on September 9, 1963
Accepted on November 29, 1963


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