HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Zilversmit, D. B. Search for Related Content PubMed PubMed Citation Articles by Zilversmit, D. B. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 5, 300-306, July 1964
Copyright © 1964 by Lipid Research, Inc.

Extraction of cholesterol from human serum lipoprotein films

D. B. Zilversmit

University of Tennessee Medical Units, Department of Physiology, Memphis, Tennessee

Serum high- and low-density lipoproteins were spread between heptane and phosphate buffer. The cholesterol from both protein films became completely soluble in the heptane phase. Other neutral lipids were also found in the heptane, but phospholipids could not be detected. Shaking of whole serum or isolated lipoproteins with heptane resulted in only slight extraction of cholesterol by the fat solvent. The addition of albumin to the lipoproteins reduced the amount of cholesterol extractable by heptane. It is postulated that when aqueous solutions of lipoproteins are shaken with nonpolar fat solvents the degree of cholesterol extraction depends on the disruption of the lipoprotein structure through contact with various interfaces, and that other proteins may protect the lipoprotein by competing for position at the interface.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?