HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH SEARCH RESULT		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gaylor, J. L. Articles by Sault, F. M. Search for Related Content PubMed PubMed Citation Articles by Gaylor, J. L. Articles by Sault, F. M. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 5, 422-431, July 1964
Copyright © 1964 by Lipid Research, Inc.

Localization and biosynthesis of 7-dehydrocholesterol in rat skin

J. L. Gaylor and Frances M. Sault

Graduate School of Nutrition, Cornell University, Ithaca, New York

By differential assay, the concentration of 7-dehydrocholesterol in the dead keratin layer, the epidermal mucosa, the sebaceous glands and associated appendages of the epidermis, and the dermis of rat skin was determined; 85% of the 7-dehydrocholesterol was isolated from slices of skin that contained surface keratin and sebaceous glands.

Slices of skin were incubated with labeled acetate in vitro. Labeled 7-dehydrocholesterol was separated from other labeled skin sterols as the hydrolyzed 5, 8 Diels-Alder adduct. Approximately 80% of the labeled 7-dehydrocholesterol was isolated from slices of rat skin that contained sebaceous glands.

The formation of cutaneous 7-dehydrocholesterol was studied in vivo by the injection of labeled acetate. More than 80% of the labeled cutaneous 7-dehydrocholesterol was isolated with the sebaceous tissue. Time-course studies suggested that ⁷-cholestenol may be a precursor of cutaneous 7-dehydrocholesterol.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. S. Blois, H. F. Blum, and W. F. Loomis Vitamin D, Sunlight, and Natural Selection Science, February 9, 1968; 159(3815): 652 - 653. [PDF]