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Journal of Lipid Research, Vol. 5, 442-447, July 1964
Copyright © 1964 by Lipid Research, Inc.

Presence of glycerokinase in guinea pig mammary gland and the incorporation of glycerol into glycerides

O. W. McBride and Edward D. Korn

Laboratory of Biochemistry, Section on Cellular Physiology, National Heart Institute, National Institutes of Health, Bethesda, Maryland

The presence of glycerokinase in homogenates of mammary tissue from lactating guinea pigs is demonstrated. It has been shown that -glycerophosphate-C¹⁴ (chromatographically identified) is formed from glycerol-1,3-C¹⁴ in the presence of homogenate. This reaction requires ATP and Mg⁺⁺. Glycerol-C¹⁴ is incorporated into the glycerol moiety of glycerides via -glycerophosphate. Glucose-C¹⁴ can also serve as a source of glyceride glycerol.

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