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Journal of Lipid Research, Vol. 5, 477-478, July 1964
Copyright © 1964 by Lipid Research, Inc.

A source of contamination in the ultramicro analysis of methyl esters of fatty acids by gas-liquid chromatography

Patricia V. Johnston and Betty I. Roots

Department of Anatomy, University College, London, England

Contaminants which could be erroneously identified as methyl esters of fatty acids on gas-liquid chromatographic (GLC) analysis were traced to anhydrous methanolic HCl used for methanolysis. Further studies indicated that the artifacts are not esters of carboxylic acids even though they mimic them on GLC analysis.

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