Journal of Lipid Research, Vol. 5, 532-537, October 1964
Copyright © 1964 by Lipid Research, Inc.

Estimation and subcellular distribution of lecithinase activity in rat intestinal mucosa

Athos Ottolenghi

Department of Physiology and Pharmacology, Duke University Medical Center, Durham, North Carolina

A method for the quantitative estimation of lecithinase has been developed using rat intestinal mucosa as the lecithinase source. Optimal conditions for lecithinase preparation and assay involve the use of buffered glycerol media with cysteamine and EDTA to stabilize the activity. Lecithin hydrolysis proceeds with apparent first order reaction kinetics when sonicated lecithin-oleate suspensions are used: in these conditions the rate of hydrolysis is proportional to the lecithinase concentration. The method gives satisfactory results in subcellular distribution studies. Fractionation by differential centrifugation and by treatment with polymixin or pH change strongly suggest that the enzyme activity is bound to ribosomal structures.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. Jiang, S. K. Noh, and S. I. Koo Egg Phosphatidylcholine Decreases the Lymphatic Absorption of Cholesterol in Rats J. Nutr., September 1, 2001; 131(9): 2358 - 2363. [Abstract] [Full Text] [PDF]