J. Lipid Res.
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Journal of Lipid Research, Vol. 5, 628-a-631, October 1964
Copyright © 1964 by Lipid Research, Inc.

Quantitative two-dimensional thin-layer chromatography of naturally occurring phospholipids

David Abramson and Melvin Blecher

Department of Biochemistry, Schools of Medicine and Dentistry, Georgetown University, Washington, D.C.

A method for the complete separation and essentially quantitative isolation of individual components of phospholipid mixtures is described. The mixture is chromatographed in two dimensions on thin layers of Silica Gel G and the phospholipids are extracted from the adsorbent with a solvent mixture containing formic acid.

Submitted on February 20, 1964
Accepted on May 13, 1964


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