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J. Lipid Res.
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Originally published In Press as doi:10.1194/jlr.R900018-JLR200 on July 3, 2009

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Journal of Lipid Research, Vol. 50, 1976-1985, October 2009
Copyright © 2009 by American Society for Biochemistry and Molecular Biology

Thematic Review

Thematic Review Series: Proteomics

Contributions of quantitative proteomics to understanding membrane microdomains

Yu Zi Zheng and Leonard J. Foster1

Centre for High-Throughput Biology and Department of Biochemistry and Molecular Biology, 2125 East Mall, University of British Columbia, Vancouver, BC, Canada, V6T 1Z4

1 To whom correspondence should be addressed. e-mail: ljfoster{at}interchange.ubc.ca

Membrane microdomains, e.g., lipid rafts and caveolae, are crucial cell surface organelles responsible for many cellular signaling and communication events, which makes the characterization of their proteomes both interesting and valuable. They are large cellular complexes comprised of specific proteins and lipids, yet they are simple enough in composition to be amenable to modern LC/MS/MS methods for proteomics. However, the proteomic characterization of membrane microdomains by traditional qualitative mass spectrometry is insufficient for distinguishing true components of the microdomains from copurifying contaminants or for evaluating dynamic changes in the proteome compositions. In this review, we discuss the contributions quantitative proteomics has made to our understanding of the biology of membrane microdomains.

Supplementary key words membrane microdomain • lipid rafts • caveolae • detergent-resistant membranes

Abbreviations: BCR, B-cell receptor; 2DGE, two-dimensional gel electrophoresis; DRM, detergent-resistant membrane; ICAT, Isotope-Coded Affinity Tags; MβCD, methyl-β-cyclodextrin; PDGF, platelet-derived growth factor; SILAC, Stable Isotope Labeling of Amino acids in Cell culture; TCR, T-cell receptor


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