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This Article Full Text Full Text (PDF) All Versions of this Article: M800543-JLR200v1 50/10/1996    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Google Scholar Articles by Suzuki-Yamamoto, T. Articles by Ishimura, K. PubMed PubMed Citation Articles by Suzuki-Yamamoto, T. Articles by Ishimura, K. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 50, 1996-2003, October 2009
Copyright © 2009 by American Society for Biochemistry and Molecular Biology

Colocalization of prostaglandin F₂ receptor FP and prostaglandin F synthase-I in the spinal cord

T. Suzuki-Yamamoto^1,*, K. Toida, Y. Sugimoto and K. Ishimura^**

^* Department of Nutritional Science, Okayama Prefectural University, Soja, Okayama 719-1197, Japan
Department of Anatomy, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan
Department of Physiological Chemistry, Graduate School of Pharmaceutical Science, Kyoto University, Kyoto 606-8501, Japan
^** Department of Anatomy and Cell Biology, Institute of Health Biosciences, University of Tokushima Graduate School, Kuramoto, Tokushima, 770-8503, Japan

¹ To whom correspondence should be addressed. e-mail: toshiko{at}fhw.oka-pu.ac.jp

Prostaglandin F₂ is synthesized by prostaglandin F synthase, which exists in two types, prostaglandin F synthase I (PGFS I) and prostaglandin F synthase II (PGFS II). Prostaglandin F₂ binds to its specific receptor, FP. Our previous immunohistochemical study showed the distinct localization of prostaglandin F synthases in rat spinal cord. PGFS I exists in neuronal somata and dendrites in the gray substance, and PGFS II exists in ependymal cells and tanycytes surrounding the central canal. Both enzymes are also present in endothelial cells of blood vessels in the white and gray substances of the spinal cord. In this study, we found that FP localizes in neuronal somata and dendrites but not in ependymal cells, tanycytes, or endothelial cells. Immunohistochemical analysis of serial sections showed the colocalization of FP and PGFS I. FP immunoreactivity was intense in spinal laminae I and II of the dorsal horn, a connection site of pain transmission, and was similar to that of PGFS I in neuronal elements. These findings suggest that prostaglandin F₂ synthesized in the neuronal somata and dendrites exert an autocrine action there.—Suzuki-Yamamoto, T., K. Toida, Y. Sugimoto, and K. Ishimura. Colocalization of prostaglandin F₂ receptor FP and prostaglandin F synthase-I in the spinal cord.

Supplementary key words immunohistochemistry • neuron • dendrite

Abbreviations: ABC, avidin-biotin complex; CLSM, confocal laser scanning microscopy; COX, cyclooxygenase; DAB, 3,3'-diamino­benzidine tetrahydrochloride; MAP2, microtubule-associated protein 2; PG, prostaglandin; PGFS, prostaglandin F synthase

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