HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M800399-JLR200v1 50/4/667    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wróblewska, M. Articles by Badzio, T. Search for Related Content PubMed PubMed Citation Articles by Wróblewska, M. Articles by Badzio, T. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 50, 667-675, April 2009
Copyright © 2009 by American Society for Biochemistry and Molecular Biology

Phospholipids mediated conversion of HDLs generates specific apoA-II pre-β mobility particles

Magorzata Wróblewska^1,*, Barbara Kortas-Stempak, Andrzej Szutowicz^* and Tadeusz Badzio

^* Department of Laboratory Medicine, Medical University of Gdask, Gdask, Poland
Department of Analytical Chemistry, Medical University of Gdask, Gdask, Poland

Published, JLR Papers in Press, December 9, 2008.

¹ To whom correspondence should be addressed. e-mail: wroblew{at}amg.gda.pl

Apolipoproteins (apo)A-I and A-II are major proteins of human HDL. The cycling of apoA-I between lipid-poor and lipid-rich forms of HDL plays a key role in the transport of cholesterol by these particles. ApoA-II resides only in part of HDL particles, and little is known about its role in HDL metabolism. Our study investigates the redistribution of apoA-II after HDL remodelling induced by exogenous phospholipids (PL). During incubation with egg yolk lecithin (EYL) liposomes, human HDL became PL-enriched and free cholesterol (FC)-depleted, and lost small amounts of apoA-I and apoA-II. The loss of FC and apolipoproteins correlated with the rise of PL content in HDL. Agarose gel electrophoresis demonstrated the appearance of new pre-β mobility fractions containing apoA-I and apoA-II in liposomes and HDL mixtures. Two-dimensional nondenaturing 2–27% PAGE has shown that the pre-β mobility fraction that appeared at initial liposome-PL/HDL-PL ratio 5:1 consisted of two distinct heterogeneous subpopulations of particles containing either apoA-I or apoA-II. Our study provides evidence that during HDL conversion mediated by PL apoA-II dissociated from HDL particles yielding apoA-II-specific pre-β mobility particles. This observation supports the hypothesis that apoA-II in plasma, like apoA-I, may cycle between lipid-poor and lipid-rich forms of HDL.

Supplementary key words apolipoprotein A-II • high density lipoproteins • A-II lipid-poor particles

Abbreviations: apo, apolipoprotein; CE, cholesteryl esters; EYL, egg yolk lecithin; FC, free cholesterol; HDL A-I, HDL containing apoA-I without apoA-II; HDL A-I/A-II, HDL containing apoA-I and apoA-II; PL, phospholipids; TC, total cholesterol

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?