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Papers In Press, published online ahead of print April 1, 2009
J. Lipid Res., doi:10.1194/jlr.M800479-JLR200

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Journal of Lipid Research, Vol. 50, 694-703, April 2009
Copyright © 2009 by American Society for Biochemistry and Molecular Biology

Plasma turnover of 3,4-didehydroretinol (vitamin A2) increases in vitamin A-deficient rats fed low versus high dietary fat^*,

Anne L. Escaron^*, Michael H. Green and Sherry A. Tanumihardjo^1,*

^* University of Wisconsin-Madison, Interdepartmental Graduate Program in Nutritional Sciences, Madison, WI
The Pennsylvania State University, Nutritional Sciences, University Park, PA

^* Supported by the International Life Sciences Institute Alex Malaspina Future Leader Award (SAT), NIHNIDDK 61973, and USDA NRI 2007-35200-17729. Part of this work was presented at the Experimental Biology Meeting in 2007, Washington, DC, by A.L.E.

The online version of this article (available at http://www.jlr.org) contains supplementary data in the form of one figure.

Published, JLR Papers in Press, November 30, 2008.

¹ To whom correspondence should be addressed. email: sherry{at}nutrisci.wisc.edu

Relationships between increased adiposity and fat-soluble vitamin storage and metabolism are poorly understood. To examine these associations, 6% or 21% dietary fat was fed to rats for 11 weeks and tissue vitamin A storage determined. Two levels of supplemental vitamin A were administered. At the end of the tenth week, 3,4-didehydroretinol (DR) was administered orally, and its kinetics were followed for 1 week in serum and tissues. Model-based compartmental analysis was applied to these data. Kidney total retinol (R) concentrations were elevated in rats fed 6% compared with 21% dietary fat (n = 24/group). The fractional transfer coefficient (FTC) describing the movement of tracer from plasma to extravascular stores was two times higher in the 6% compared with the 21% fat group. Consistent with the elevated renal R in 6% fat fed rats, there was a 2-fold increase in the FTC representing tracer distribution from plasma to kidney in the 6% compared with 21% fat group. Taken together with a fat main effect on renal vitamin A, our data support the evidence that faster turnover of kidney R may help set the mechanism governing vitamin A tissue distribution during deficiency. Rats fed 21% versus 6% dietary fat conserved hepatic R more efficiently.

Supplementary key words kinetic analysis • retinol • Windows version of Simulation, Analysis, and Modeling software • adipose tissue

Abbreviations: DR, 3,4-didehydroretinol; DRE, 3,4-didehydroretinyl esters; FTC, fractional transfer coefficient; R, retinol; RBP, retinol-binding protein; RE, retinyl esters

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