HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Papers In Press, published online ahead of print May 1, 2009
J. Lipid Res., doi:10.1194/jlr.M800658-JLR200

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M800658-JLR200v1 50/5/988    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zheng, Y. Z. Articles by Foster, L. J. Search for Related Content PubMed PubMed Citation Articles by Zheng, Y. Z. Articles by Foster, L. J. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 50, 988-998, May 2009
Copyright © 2009 by American Society for Biochemistry and Molecular Biology

Mitochondria do not contain lipid rafts, and lipid rafts do not contain mitochondrial proteins

Yu Zi Zheng, Kyra B. Berg and Leonard J. Foster¹

Centre for High-Throughput Biology and Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada, V6T 1Z4

The online version of this article (available at http://www.jlr.org) contains supplementary data in the form of eight tables.

Infrastructure used in this project was supported by the Canadian Foundation for Innovation, the British Columbia Knowledge Development Fund, and the Michael Smith Foundation through the British Columbia Proteomics Network. L.J.F. is the Canada Research Chair in Organelle Proteomics and a Michael Smith Foundation Scholar. K.B.B. was supported by a British Columbia Proteomics Network Training Award.

Published, JLR Papers in Press, January 9, 2009.

¹ To whom correspondence should be addressed. e-mail: ljfoster{at}interchange.ubc.ca

Lipid rafts are membrane microdomains involved in many cellular functions, including transduction of cellular signals and cell entry by pathogens. Lipid rafts can be enriched biochemically by extraction in a nonionic detergent at low temperature, followed by floatation on a sucrose density gradient. Previous proteomic studies of such detergent-resistant membranes (DRMs) are in disagreement about the presence of mitochondrial proteins in raft components. Here, we approach the status of mitochondrial proteins in DRM preparations by employing stable isotope labeling by amino acids in cell culture to evaluate the composition of differentially purified subcellular fractions as well as high-resolution linear density gradients. Our data demonstrate that F₁/F₀ ATPase subunits, voltage-dependent anion selective channels, and other mitochondrial proteins are at best partially copurifying contaminants of raft preparations.

Supplementary key words detergent-resistant membranes • membrane proteins • stable isotope labeling by amino acids in cell culture • quantitative proteomics • mass spectrometry • methyl-β-cyclodextrin • protein correlation profiling

Abbreviations: DRMs, detergent-resistant membranes; LC-MS/MS, liquid chromatography-tandem mass spectrometry; MβCD, methyl-β-cyclodextrin; MBS, MES-buffered saline; PC, principal component; PCA, principal component analysis; PCP, protein correlation profiling; r.c.f., relative centrifugal force; DMEM, Dulbecco's Modified Eagle's Medium; RPMI, Roswell Park Memorial Institute; SDC, sodium deoxycholate; SILAC, stable isotope labeling by amino acids in cell culture; VDAC, voltage-dependent anion selective channel; WCM, whole-cell membrane

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Osman, C. Merkwirth, and T. Langer Prohibitins and the functional compartmentalization of mitochondrial membranes J. Cell Sci., November 1, 2009; 122(21): 3823 - 3830. [Abstract] [Full Text] [PDF]

				Y. Z. Zheng and L. J. Foster Contributions of quantitative proteomics to understanding membrane microdomains J. Lipid Res., October 1, 2009; 50(10): 1976 - 1985. [Abstract] [Full Text] [PDF]