HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Papers In Press, published online ahead of print September 1, 2009
J. Lipid Res., doi:10.1194/jlr.M900047-JLR200

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M900047-JLR200v1 50/9/1776    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Sugiura, Y. Articles by Setou, M. Search for Related Content PubMed PubMed Citation Articles by Sugiura, Y. Articles by Setou, M. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 50, 1776-1788, September 2009
Copyright © 2009 by American Society for Biochemistry and Molecular Biology

Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry^[S]

Yuki Sugiura^*,,, Yoshiyuki Konishi^,, Nobuhiro Zaima^,, Shigeki Kajihara, Hiroki Nakanishi^**,, Ryo Taguchi^**, and Mitsutoshi Setou^1,,

^* Department of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan
Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan
Technology Research Laboratory, Shimadzu Corporation, 3-9-4, Hikaridai, Seika-cho, Souraku-gun, Kyoto 619-0237, Japan
^** Department of Metabolome, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongou, Bunkyo-ku, Tokyo 113-0033, Japan
Core Research for Evolutional Science and Technology, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan
Department of Molecular Anatomy, Hamamatsu University School of Medicine, Handayama 1-20-1, Hamamatsu, Shizuoka 431-3192, Japan

¹ To whom correspondence should be addressed. e-mail: setou{at}hama-med.ac.jp

Previous studies have shown that MALDI-imaging mass spectrometry (IMS) can be used to visualize the distribution of various biomolecules, especially lipids, in the cells and tissues. In this study, we report the cell-selective distribution of PUFA-containing glycerophospholipids (GPLs) in the mouse brain. We established a practical experimental procedure for the IMS of GPLs. We demonstrated that optimization of the composition of the matrix solution and spectrum normalization to the total ion current (TIC) is critical. Using our procedure, we simultaneously differentiated and visualized the localizations of specific molecular species of GPLs in mouse brain sections. The results showed that PUFA-containing phosphatidylcholines (PCs) were distributed in a cell-selective manner: arachidonic acid- and docosahexaenoic acid-containing PCs were seen in the hippocampal neurons and cerebellar Purkinje cells, respectively. Furthermore, these characteristic localizations of PUFA-PCs were formed during neuronal maturation. The phenomenon of brain cell-selective production of specific PUFA-GPLs will help elucidate the potential physiological functions of PUFAs in specific brain regions.

Supplementary key words MALDI • imaging MS • LC ESI-MS/MS • phospholipids • development

Abbreviations: AA, arachidonic acid; DHA, docosahexaenoic acid; DHB, dihydroxybenzoic acid; GPL, glycerol phospholipid; IMS, imaging mass spectrometry; ITO, indium-tin-oxide; PC, phosphatidylcholine; PCA, principle component analysis; PLA₂, phospholipase A₂; QIT, quadrupole ion trap; SIMS, secondary IMS; TIC, total ion current; TOF, time-of-flight

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?