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Journal of Lipid Research, Vol. 51, 824-831, April 2010
Copyright © 2010 by American Society for Biochemistry and Molecular Biology

Quantitation of serum angiopoietin-like proteins 3 and 4 in a Finnish population sample

Marius R. Robciuc^*, Esa Tahvanainen, Matti Jauhiainen^* and Christian Ehnholm^1,*

^* Department of Chronic Disease Prevention, National Institute for Health and Welfare, Public Health Genomics Research Unit, Biomedicum, Helsinki, Finland
Department of Medical Genetics, University of Helsinki, Helsinki, Finland

¹ To whom correspondence should be addressed. e-mail: christian.ehnholm{at}thl.fi

We have developed and validated quantitative ELISAs for human angiopoietin-like (ANGPTL)3 and 4 and correlated their serum levels with parameters of lipid and carbohydrate metabolism. For this study, we used a random subsample of the Health 2000 Health Examination Survey consisting of 125 men and 125 women, aged 30–94 years. The anthropometric and biochemical parameters of subjects were characterized in detail. ANGPTL 3 and 4 levels were determined using the developed ELISAs. The intra- and inter-assay coefficients of variation for the assays were less than 15%. The average serum concentration of ANGPTL3 was 368 ± 168 ng/ml (mean ± SD) and for ANGPTL4 it was 18 ± 23 ng/ml (mean ± SD). ANGPTL4 serum levels displayed high variability between individuals ranging from 2 to 158 ng/ml. In post-heparin plasma, both ANGPTL 3 and 4 were increased. Low levels of ANGPTL3 were associated with decreased HDL-cholesterol and increased triglyceride levels. ANGPTL4 levels were positively correlated with FFAs (P = 0.044) and waist-hip ratio (P = 0.016). The developed ELISAs will be important tools to clarify the role of ANGPTL 3 and 4 in human energy metabolism and partitioning of triglycerides between sites of storage (adipose tissue) and oxidation (skeletal and cardiac muscle).

Supplementary key words ELISA • lipoproteins • triacylglycerols • high density lipoproteins • body weight • body mass index • serum lipids • post-heparin plasma

Abbreviations: ANGPTL, angiopoietin-like; apo, apolipoprotein; BMI, body mass index; CRP, c-reactive protein; EL, endothelial lipase; Hcy, homocysteine; HOMA IR, homeostasis model assessment for insulin resistance; PHP, post-heparin plasma; TC, total cholesterol; TG, triglyceride

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