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Journal of Lipid Research, Vol. 6, 10-15, January 1965
Copyright © 1965 by Lipid Research, Inc.

A stereospecific analysis of triglycerides

H. Brockerhoff

Fisheries Research Board of Canada, Technological Research Laboratory, Halifax, Nova Scotia, Canada

A method is presented for the analysis of the fatty acid compositions in each of the three positions of a triglyceride. Diglycerides are obtained from the triglyceride by lipolysis with pancreatic lipase, and converted to a mixture of d- and l-phosphatidyl phenol. The l-phosphatide is then hydrolyzed by phospholipase A, leaving a lysophosphatide with the fatty acid in position 1, free fatty acid from position 2, and unhydrolyzed d-phosphatidyl phenol. The fatty acid composition in position 3 of the original triglyceride is obtained by calculation.

The method was tested on two triglyceride mixtures with known fatty acid distributions. A natural triglyceride, corn oil, was analyzed. The distribution of the major fatty acids between positions 1 and 3 was found to be nearly random.

Supplementary key words stereospecific analysis • triglyceride analysis • triglyceride structure • corn oil • lipolysis • phospholipase A • phosphatidyl phenol • lysophosphatide phenol • fatty acid distribution

Submitted on April 27, 1964
Accepted on August 3, 1964

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