HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kopaczyk, K. C. Articles by Radin, N. S. Search for Related Content PubMed Articles by Kopaczyk, K. C. Articles by Radin, N. S. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 6, 140-145, January 1965
Copyright © 1965 by Lipid Research, Inc.

In vivo conversions of cerebroside and ceramide in rat brain

Krystyna C. Kopaczyk and Norman S. Radin

Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan

Lignoceric acid-1-C¹⁴ was used to make lignoceroyl psychosine (kerasin) and stearic acid-1-C¹⁴ to make stearoyl sphingosine (ceramide). Emulsions of the labeled sphingolipids were injected directly into the brains of young rats and the resultant lipoidal metabolic products were examined for radioactivity.

Most of the metabolized C¹⁴ was found in the ester-linked fatty acids, particularly in the acids corresponding to the ones injected. This shows that the lignocerate and stearate were released from amide linkage and incorporated into other lipids. Activity was found also in other fatty acids and cholesterol, showing that the released fatty acids were partially degraded to acetate. Ceramide and sphingomyelin showed relatively high activities, and the fatty acids of these lipids were labeled primarily in the carboxyl group. This suggests that ceramide is formed directly from cerebroside via a galactosidase, and that the ceramide is converted to free fatty acids as well as sphingomyelin. Little activity was found in the hydroxy cerebrosides and sulfatides, suggesting that neither of the injected lipids is directly converted to these glycolipids. Ester-linked lignocerate was found to occur naturally in brain.

Supplementary key words cerebroside • ceramide • brain metabolism • rat • lignoceroyl-1-C¹⁴ cerebroside • stearoyl-1-C¹⁴ psychosine • stearoyl-1-C¹⁴ ceramide • intracranial injection • metabolic conversions • sphingomyelin biosynthesis • cerebroside sulfate

Submitted on July 20, 1964
Accepted on September 22, 1964

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Terayama, T. Seiki, A. Nakamura, K. Matsumori, S. Ohta, S. Oka, M. Sugita, and T. Kawasaki Purification and Characterization of a Glucuronyltransferase Involved in the Biosynthesis of the HNK-1 Epitope on Glycoproteins from Rat Brain J. Biol. Chem., November 13, 1998; 273(46): 30295 - 30300. [Abstract] [Full Text] [PDF]

				K. M. Koshy and J. M. Boggs Investigation of the Calcium-mediated Association between the Carbohydrate Head Groups of Galactosylceramide and Galactosylceramide I^3 Sulfate by Electrospray Ionization Mass Spectrometry J. Biol. Chem., February 16, 1996; 271(7): 3496 - 3499. [Abstract] [Full Text] [PDF]

				N. Ueda, Y. Kurahashi, S. Yamamoto, and T. Tokunaga Partial Purification and Characterization of the Porcine Brain Enzyme Hydrolyzing and Synthesizing Anandamide J. Biol. Chem., October 6, 1995; 270(40): 23823 - 23827. [Abstract] [Full Text] [PDF]

				M. Sugita, J. T. Dulaney, and H. W. Moser Ceramidase Deficiency in Farber's Disease (Lipogranulomatosis) Science, December 8, 1972; 178(4065): 1100 - 1102. [Abstract] [PDF]

				A. Rodwell Fatty-Acid Composition of Mycoplasma Lipids: Biomembrane with Only One Fatty Acid Science, June 21, 1968; 160(3834): 1350 - 1351. [Abstract] [PDF]