J. Lipid Res.
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Journal of Lipid Research, Vol. 6, 157-159, January 1965
Copyright © 1965 by Lipid Research, Inc.

Photometric adaptation of Dole's microdetermination of free fatty acids

Frederick Mosinger

Laboratory of Metabolic Regulation, Institute of Human Nutrition, Prague, Czechoslovakia

The method is based on the measurement of color changes in phenol red barbital buffer in heptane-ethanol. It has been applied to human serum and to incubation media containing fatty acids liberated from rat epididymal adipose tissue. The values obtained were found to agree with those using the titrimetric method, within the limit of experimental error.

Supplementary key words free fatty acids • determination • colorimetry • serum • incubation media

Submitted on July 6, 1964
Accepted on September 2, 1964


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