Journal of Lipid Research, Vol. 6, 159-162, January 1965
Copyright © 1965 by Lipid Research, Inc.
Separation of long-chain bases by thin-layer chromatography; instability of sphingosine
Benjamin Weiss and Richard L. Stiller
Department of Biochemistry, New York State Psychiatric Institute and College of Physicians and Surgeons, Columbia University, New York, N.Y.
Purified sphingosine was resolved into dihydrosphingosine, sphingosine, and a fast-moving component by thin-layer chromatography on Silica Gel G. The fast-moving component was generated by the adsorbent, and consisted of a mixture of the cationic forms of dihydrosphingosine and sphingosine. Other sphingosine derivatives gave rise to similar artifacts.
Sphingosine formed two degradation products when stored in solution or on thin-layer plates; related long-chain bases with some of their reactive sites blocked did not deteriorate when stored in solution.
Supplementary key words thin-layer chromatography • sphingosine • dihydrosphingosine • O-methyl sphingosines • N-acyl sphingosines • hydrochlorides • cationic forms • instability • tribenzoyl sphingosines
Submitted on July 20, 1964
Accepted on October 5, 1964
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