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Journal of Lipid Research, Vol. 6, 27-32, January 1965
Copyright © 1965 by Lipid Research, Inc.

Uptake and metabolism of retinol (vitamin A) in the isolated perfused rat liver

Richard D. Zachman and James Allen Olson

Department of Biochemistry, University of Florida College of Medicine, Gainesville, Florida

The isolated perfused liver was employed to study various aspects of retinol-C¹⁴ metabolism. The rate of uptake of retinol, retinal, retinoic acid, and retinol acetate was rapid (first-order rate constant, k = 0.12-0.16 min ^-1) during the first 3-10 min of perfusion, and then decreased quickly. After 1 hr of perfusion, 50-60% of perfusate retinal-C¹⁴, 25-30% of retinol-C¹⁴, and 20% of retinol-C¹⁴ acetate were found in the liver as retinol-C¹⁴ ester, and about 10% of the original perfusate radioactivity appeared in the bile as a group of water-soluble metabolites. The amount of retinol ester formed in the liver during 1 hr was linearly dependent on the concentration of retinol in the perfusate over the range of 3 to 1000 µg/100 ml. Serum, bile salt, or prior injections of India ink had no apparent effect on the formation and storage of retinol ester during perfusion. Only the water-soluble metabolites, but not retinol and its associated derivatives, were released into the perfusate and bile. After 1 hr of perfusion, about 20% of the retinoic acid-C¹⁴ was found in the liver as free retinoic acid, and 5% was present in a nonionic fraction.

Supplementary key words Vitamin A-C¹⁴ • retinol-C¹⁴ • retinoic acid • retinal • uptake • metabolism • rat • liver perfusion • bile • bile metabolite • serum • esterification • Tween 80

Submitted on June 5, 1964
Accepted on August 19, 1964

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