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Journal of Lipid Research, Vol. 6, 3-6, January 1965
Copyright © 1965 by Lipid Research, Inc.

Separation of glycosyl diglycerides from phosphatides using silicic acid column chromatography

Marie L. Vorbeck and G. V. Marinetti

Department of Biochemistry, The University of Rochester, School of Medicine and Dentistry, Rochester, New York

A procedure has been developed for the separation of the glycosyl diglycerides from the phosphatides of Gram-positive bacteria on columns of silicic acid. The method utilizes mixtures of acetone in chloroform for elution of the glycosyl diglycerides, followed by increasing amounts of methanol in chloroform for elution of the phosphatides. The course of the fractionation was followed by means of phosphorus and carbohydrate determinations and by paper chromatography. The completeness of the separation of the phosphatides from the sugar-containing lipids was shown also by chromatographing a total lipid extract containing P³²-labeled phosphatides.

Supplementary key words phosphatides • glycolipids • bacterial lipids • glycosyl diglycerides • silicic acid chromatography • separation of lipid classes

Submitted on July 6, 1964
Accepted on August 31, 1964

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