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Journal of Lipid Research, Vol. 6, 33-36, January 1965
Copyright © 1965 by Lipid Research, Inc.

Biosynthesis of 5-cholestan-3ßbeta;-ol in rat and guinea pig liver in vitro

Sarah Shefer , Susan Milch , and Erwin H. Mosbach

Department of Laboratory Diagnosis, Public Health Research Institute of the City of New York, Inc., and the Bureau of Laboratories, New York City Department of Health, New York, N. Y.

The in vitro conversion of dl-mevalonate-2-C¹⁴ and cholesterol-4-C¹⁴ to 5-cholestan-3ßbeta;-ol was studied in liver homogenates of rats and guinea pigs. On the average 0.01-0.05% of the substrate radioactivity was converted to 5-cholestan-3ßbeta;-ol by the livers of both species during the 4 hr incubation period. The biosynthesis of 5-cholestan-3ßbeta;-ol by cell-free liver homogenates in vitro demonstrates that the presence of this saturated sterol is at least partly due to biosynthesis by mammalian tissues, and cannot be ascribed exclusively to bacterial action upon cholesterol in the intestine.

Supplementary key words 5-cholestan-3ßbeta;-ol • biosynthesis • in vitro • liver homogenate • rat • guinea pig • adrenal • mevalonate-2-C¹⁴ • cholesterol-4-C¹⁴ • triparanol

Submitted on April 16, 1964
Accepted on August 6, 1964

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